This instrument combines the best of quadrupole, ion trap and orbitrap mass analysis. In mass spectrometry, orbitrap is an ion trap mass analyzer consisting of an outer barrellike electrode and a coaxial inner spindlelike electrode that traps ions in an orbital motion around the spindle. Great ux mmass is designed to be feature rich, yet still easy to use. Ions generated by api are collected in the ltq xl followed by axial ejection to the cshaped storage trap which is used to store and collisionally. While the orbitrap is known for its high mass resolution, it is also quite good in terms of mass accuracy. Mass spectrometry for proteomics part one duration. The program is specifically designed for use with orbitrap fusion data, and is also tested to be fully compatible with the ltq orbitrap and q exactive plus. It incorporates a second ion source ris, which is located in the api source interface just after the electrodynamic ion funnel. Faster, more powerful speed productivity confidence the thermo scientific q exactive hf hybrid quadrupoleorbitrap mass spectrometer features an ultrahighfield orbitrap analyzer which doubles its speed and resolution more samples run and amazing data quality. Thanks to its orbitrap mass analyzer, the exactive plus system delivers. Hybrid ion trap orbitrap mass spectrometry creative. Potential of orbitrap mass spectrometry for application in.
An orbitrap is an ion trap mass analyzer consisting of an outer barrellike electrode and a coaxial inner spindlelike electrode that traps ions in an orbital motion around the spindle. Video on compound discoverer software for q exactive hram data. Fundamentals and applications of fourier transform mass spectrometry is the first book to delve into the underlying principles on the topic and their linkage to industrial applications. The hybrid linear ion trap orbitrap ms is one of fourier transform based hybrid instruments, while the first mass analyzer is a linear ion trap lit, and the second one is high resolution orbitrap. Although the orbitrap mass analyzer is rightfully considered to be one of the. Performance of orbitrap mass analyzer at various space. Introduction to thermo scientifictm orbitrap hrms technology. Thermo scientific ltq orbitrap velos hy b rid ft mass. Which q exactive orbitrap mass spectrometer is best for my lab. Using the principle of orbital trapping in electrostatic fields,8 the orbitrap consists of an inner and an outer electrode, which are shaped to create a quadrologarithmic electrostatic potential.
The roots of the orbitrap analyzer can be traced back to 1923 when the principle of. Metabolome identification using lcmsn orbitrap based mass spectrometry. Since 2008, a standalone orbitrap mass spectrometer, exactive, has been built, wherein the ion source was directly linked to the ctrap. The orbitrap is a new mass spectrometer based on a new type of mass analyzer invented by makarov. Ltq orbitrap xl offering outstanding mass accuracy, resolving power, dynamic range, and high sensitivity the hybrid ft mass spectrometer combines a linear ion trap ms and the orbitrap mass analyzer. So, this peptide gets fragmented into various pieces as you can see here where again mass to charge ratio over the intensity. Bailey 3, and jonathan josephs 3 thermo fisher scienti c, 1 im steingrund 46, dreieich, germany, 2 hannakunathstr. Tribrid architecture includes a quadrupole mass filter, linear ion trap and an orbitrap mass analyzer for maximal duty cycle due to a high degree of parallelization of various instrument operations. The orbitrap fusion lumos tribrid mass spectrometer with 1m option offers ultrahigh resolution 1,000,000 fwhm at mz 200 in the highest performance system. Fundamentals and applications of fourier transform mass. Once trapped, the ions orbit around the central filament and also oscillate in the horizontal z direction. The orbitrap is an ion trap mass analyzer that consists of two outer electrodes and a central electrode, which enable it to act as both an analyzer and detector.
Most advanced mass spectrometer for the most advanced research applications excel at the most challenging of applications, including low level ptm analysis, multiplexed relative quantitation using isobaric tags, intact protein characterization, as well as ms n analysis of small molecules with the new thermo scientific orbitrap fusion lumos tribrid mass spectrometer. The image current from the trapped ions is detected and converted to a mass spectrum using the fourier transform of the frequency signal. So, first mass analyzer isolates this masstocharge ratio and sends that on for fragmentation and second, a second mass spec analysis. Conclusions theory predicts that orbitrap mass analyzers will lose resolution at higher mz.
A new benchtop mass spectrometer has been developed based on an api ion source combined with a standalone orbitrap mass analyzer. The key attributes of this analyzer are accurate mass and high resolution similar to those achievable. With its wide dynamic range the q exactive biopharma platform on the q exactive plus and hf instruments provide superior peptide mapping performance within the most complex samples. Jun 14, 20 thermo orbitrap fusion animation jamie humphries. David peake, ioanna ntai, amanda souza and ralf tautenhahn. In addition to describing the orbitrap mass analyzer, this paper also describes a complete orbitrapbased mass spectrometer, equipped with an electrospray ionization source esi. Thermo scientifi orbitrap fusion lumos tribrid mass. Stay focused on your data interpretation, not on the software. Sep 07, 2012 targeted protein analysis with triple quadrupole mass spectrometers keeps on showing significant superiority for such samples. Can anyone help with an orbitrap mass spectrometer. Targeted proteomic quantification on quadrupoleorbitrap. Using the principle of orbital trapping in electrostatic fields,8 the orbitrap consists of an inner and an outer electrode, which are shaped to create a. Fourier transform ion cyclotron resonance an overview.
Injection at a point displaced from z 0 gives the ions potential energy in the zdirection. In a quadrupole mass spectrometer the quadrupole is the mass analyzer the component of the. The litorbitrap combination can be used for both ms and msn experiments because the lit itself is a detector. Ions are transferred from the esi source through three stages of differential pumping using rf guide quadrupoles. Orbitrap ms is a relatively new technology, and able to perform untargeted. In contrast to other existing mzxml conversion utilities, rthur uses thermo foundation 3. The attractive features of the quadrupole as a mass analyzer are evident from the above discussion.
Modern orbitrap instruments have mass accuracies in the range of 15 ppm. The ltqorbitrap hybrid mass spectrometer is a high mass accuracy, high resolution mass analyzer, capable of achieving resolving power 100,000 and having mass accuracy. The orbital trap mass analyzer provides a number of unique analytical features along with inevitable limitations as an electrostatic instrument operating in high space charge regimes resulting in systematic measured frequency errors as an effect of stored ion clouds on the trap field and each other effect of nonideal machining the trap electrodes, effect of injection slot, effect of real. Mass spectrometrybased proteomics using q exactive, a high. The ions are injected with a velocity perpendicular to the long axis of the orbitrap the zaxis. Superior resolution of agilent 6540 uhd qtof over thermo ltq. An orbitrap fusion lumos ms with the internal calibration configuration significantly improves the mass tocharge ratio mz assignment accuracy to less than 1 ppm. The power of the multiawardwinning orbitrap gcms technology has so far allowed research scientists to break new ground and gain a broader and deeper understanding of their samples through the use of highresolution, accurate mass hram analysis. Employing a simple lockmass scheme can reduce mass errors to less than 2 ppm olsen et al. Orbitrap intruments 16 the orbitrap is an entirely new type of mass analyzer invented by marakov in 1999. Ions are injected into the orbitrap at the point indicated by the red arrow. Performance evaluation of a highfield orbitrap mass analyzer. The quadrupole mass analyzer qms is one type of mass analyzer used in mass spectrometry. Thermo fisher orbitrap orbitrap fusion planet orbitrap.
The unmatched mass accuracy and resolution achieved by the orbitrap mass analyzer allows full sequence coverage to be achieved confidently, each and every time. Performance evaluation of a highfield orbitrap mass analyzer alexander makarov, eduard denisov, and oliver lange thermo fisher scientific, bremen, germany a new design of the orbitraptm mass analyzer is presented. Orbitrap ms is a relatively new technology, and able to. Targeted proteomic quantification on quadrupoleorbitrap mass. Timeofflight from the ctrap to the orbitrap is proportional to mz 12 2 while axial frequency is proportional to mz12,so the phase acquired by ions traveling along effective length. This reduction in resolution with mass makes it more difficult to distinguish highermass analytes from coeluting sample components. The orbitrap mass analyzer, the subject of this paper, bears a similarity to an earlier ion storage device, the kingdon trap, as well as to two types of iontrapping mass analyzers, the paul trap quadrupole ion trap, and the fourier transform ion cyclotron resonance instrument. This reduction in resolution with mass makes it more difficult to distinguish higher mass analytes from coeluting sample components. Thermo scientific orbitrap fusion tribrid mass spectrometer.
Oct 03, 2016 the sensitivity, selectivity, flexibility, and easeofuse provided by hybrid quadrupole orbitrap ms set the standard for screening, quantitation, identification, and confirmation of targeted and. Orbitrap mass analyzer incredible resolving power up to 120,000 fwhm for the q exactive gc at mz 200. Enabling mass spectrometric analysis of intact proteins in native conditions on a hybrid quadrupoleorbitrap mass spectrometer hmr mode kai schef er 1, eugen damoc 2, aaron o. The invention of the orbitrap analyzer and its proofofprinciple by makarov at the end of the. Built on the proven performance of the q exactive plus platform, the q. The thermo scientific orbitrap fusion tribrid mass spectrometer.
Dec 15, 2006 please be advised that we experienced an unexpected issue that occurred on saturday and sunday january 20th and 21st that caused the site to be down for an extended period of time and affected the ability of users to access content on wiley online library. The orbital trap mass analyzer provides a number of unique analytical features. In malditof mass spectrometry, the ion source is matrixassisted laser desorptionionization maldi, and the. Metabolomics core facility instrumentation and software embl. White paper on high resolution accurate mass hram with an orbitrap mass analyzer. Superior resolution of agilent 6540 uhd qtof over thermo. The thermo scientific orbitrap fusion tribrid mass spectrometer combines the best of quadrupole, orbitrap, and ion trap mass analysis in a revolutionary tribrid architecture that delivers unprecedented depth of analysis. Orbitrap eclipse tribrid mass spectrometer thermo fisher. Enables better sensitivity improved signalnoise ratio and performs better mass measurements of intact proteins at higher resolution. Development and applications of a new benchtop orbitrap. Thermo ltq orbitrap xl also diminishes with mass, and at 5 spectrasecond is only about 4000 at mz values around 1800. Mass spectrometry and fourier transform infrared spectroscopy for analysis of biological materials by timothy james anderson a dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of doctor of philosophy major.
The thermo scientific ltq orbitrap velos combines the proven mass accuracy and ultra high resolution of the orbitrap mass analyzer, with the increased s ensitivity and improved cycle time of the ltq velos 7khkljk mass accuracy of the ltq orbitrap velos increases the speed and confidence of identification in complex. In mass spectrometry, orbitrap is an ion trap mass analyzer consisting of an outer barrellike. The general principle of most search engines is the selection of. Although a wholly new mass analyzer, for didactic purposes it is useful to consider the orbitrap as a modi. Advanced hybrid quadrupoleorbitrap mass spectrometer with high resolution up to 280,000, fast scan speed and hcd fragmentation. In malditof mass spectrometry, the ion source is matrixassisted laser desorptionionization maldi, and the mass analyzer is timeofflight tof analyzer. The orbitrap fusion systems nextgeneration hardware and software make setup easier. Ions are injected off center into a radial spindlelike dconly trap. Subppm mass accuracy with a high acquisition rate, driving unrivaled spectral quality. Faster, more powerful speed productivity confidence the thermo scientific q exactive hf hybrid quadrupole orbitrap mass spectrometer features an ultrahighfield orbitrap analyzer which doubles its speed and resolution more samples run and amazing data quality. Performance evaluation of a hybrid linear ion traporbitrap. Ultrahigh resolving power up to 500,000 fwhm, with isotopic fidelity up to 240,000 fwhm at mz 200. The image current from the trapped ions is detected and converted to a mass spectrum using. In addition to describing the orbitrap mass analyzer, this paper also describes a complete orbitrap based mass spectrometer, equipped with an electrospray ionization source esi.
It enables life scientists analyzing even the most challenging lowabundance, highcomplexity, or difficult samples to identify more compounds more quickly. Orbitrap mass spectrometer,orbitrap mass spectrometer. Development and applications of a new benchtop orbitrap mass. Orbitrap mass analyzer encyclopedia of genetics, genomics. It enables life scientists analyzing even the most challenging lowabundance, highcomplexity, or difficult. Performance of orbitrap mass analyzer at various space charge. Instructions are available to fix an issue with the etd reagent intensity on orbitrap fusion only systems. Thermo fisher orbitrap orbitrap fusion lumos 1m planet orbitrap. In mass spectrometry, orbitrap is an ion trap mass analyzer consisting of an outer barrellike electrode and a coaxial inner spindlelike electrode that traps ions. Ions are injected into the trap at the point indicated by the red arrow.
Overview of the easyic option superior mass accuracy in every scan an orbitrap fusion lumos ms with the internal calibration configuration significantly improves the mass tocharge ratio mz assignment accuracy to less than 1 ppm. Orbitrap cutaway view of the orbitrap mass analyzer. Platform independent no matter what operating system you are using, mmass works on ms windows, apples mac os x and linux platforms as well. Orbitrap mass spectrometry ms could lead to improved analytical performance of the current nbs by reducing the numbers of false positives and negatives, and the simultaneous measurement of confirmative secondary targets. From hu q, noll rj, li h, makarov a, hardman m, cooks g. Hybrid ion trap orbitrap mass spectrometry creative proteomics. Crosssection of the ctrap and orbitrap analyzer ion optics and di.
Enabling mass spectrometric analysis of intact proteins in. In the first mode, the orbitrap mass analyzer acquires the full ms spectra and the ltq. Improves orbitrap mass analyzer performance for everyday proteomics applications and more. Ions entering the orbitrap are captured through electrodynamic squeezing, after which they oscillate around the central electrode and in between the two outer electrodes. Mass spectrometry is an analytical technique in which samples are ionized into charged molecules and ratio of their masstocharge mz can be measured.
The same factors that distort ion trajections and mass resolution are the same issues that affect mass accuracy. As the name implies, it consists of four cylindrical rods, set parallel to each other. Upgrade your maps peptide mapping thermo scientific. The five main characteristics for measuring the performance of a mass analyzer are 1 the mass range limit or dynamic range 2 the analysis speed u ms1 3 the transmission no.
Relative quantitation of tmtlabeled proteomes focus on. The ultrahigh resolution along with the versatility of the tribrid platform with unique ms n capability and a multitude of fragmentation modes allows for comprehensive characterization. Thermo fisher orbitrap orbitrap fusion lumos easyic. It is also known as a transmission quadrupole mass spectrometer, quadrupole mass filter, or quadrupole mass spectrometer. Low voltage safety compliance this device complies with low voltage directive 200695ec and harmonized standard en 610101. Thermo fisher orbitrap exactive plus planet orbitrap. The orbitrap mass analyzer is proving itself as a useful addition to a proteomics tool box. Linear trap quadropole ltq orbitrap velos mass spectrometry. The lit orbitrap combination can be used for both ms and msn experiments because the lit itself is a detector. Improved accumulation and transmission of high molecular weight ions to the orbitrap mass analyzer. Q exactive orbitrap mass spectrometers thermo fisher scientific. The orbitrap exploris 480 mass spectrometer joins the family of nextgeneration thermo scientific mass spectrometers built on a common architecture with the guiding principle of ultimate performance and ease of use.
International journal of mass spectrometry 254 2006 5362 ion trajectory simulations of axial ac dipolar excitation in the orbitrap g. Because of the shape of the spindle and the off center injection, the ions obtain a velocity component in the z direction. The orbitrap fusion lumos tribrid mass spectrometer with 1m option offers ultrahigh resolution. Orbitrap exploris mass spectrometers thermo fisher. Innovative software and instrument design let you concentrate on your. The sensitivity, selectivity, flexibility, and easeofuse provided by hybrid quadrupole orbitrap ms set the standard for screening, quantitation, identification, and confirmation of targeted and.
516 1391 241 940 716 628 1046 932 1126 1296 577 183 1510 1535 953 1392 336 114 368 1521 88 1392 175 305 618 1362 775 733 538 366 142 1141 331 1059 394 364 1477 630 793 1097